Journal: STAR Protocols
Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells
doi: 10.1016/j.xpro.2026.104519
Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
Article Snippet: CD44 Antibody, anti-mouse, PE-Vio 770 clone IM7.8.1; 1:10 dilution , Miltenyi Biotec , Cat# 130-102-377 RRID: AB_2658187.
Techniques: Cell Culture, Dot Blot, Luminex